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Polyclonal antibody production schedule in rodent Week 0 Bleed pre-immune serum, 0.2 ml for mouse and 0.5 ml for rat Immunize with 25-50 µg/rabbit antigen in Complete Freund's Adjuvant . Week 2 Immunize with 15-25 µg antigen in Incomplete Freund's Adjuvant (IFA). Week 4 Immunize with 15-25 µg antigen in Incomplete Freund's Adjuvant (IFA). Week 6 Test bleed 0.2-0.5 ml. Immunize with 15-25 µg antigen in Incomplete Freund's Adjuvant (IFA). Test for positive production (ELISA, Western, etc.) Week 8 Immunize with 10-15 µg antigen in Incomplete Freund's Adjuvant (IFA). Week 10 Production bleed. Continue production bleed every four weeks.